OPTIMATION OF DNA EXTRACTION PROTOCOL OF Elaeidobius kamerunicus

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Sri Wening
Agus Eko Prasetyo
Tjut Ahmad Perdana Rozziansha
Agus Susanto

Abstract

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,
material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.

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How to Cite
Wening, S., Prasetyo, A. E., Rozziansha, T. A. P., & Susanto, A. (2016). OPTIMATION OF DNA EXTRACTION PROTOCOL OF Elaeidobius kamerunicus. Jurnal Penelitian Kelapa Sawit, 24(2), 77-86. https://doi.org/10.22302/iopri.jur.jpks.v24i2.10
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Articles
Author Biographies

Sri Wening, Indonesian Oil Palm Research Institute (IOPRI)

Breeding & Plant Biotechnology Reserach Group

Agus Eko Prasetyo, Indonesian Oil Palm Research Institute (IOPRI)

Plant Protection Research Group

Tjut Ahmad Perdana Rozziansha, Indonesian Oil Palm Research Institute (IOPRI)

Plant Protection Research Group

Agus Susanto, Indonesian Oil Palm Research Institute (IOPRI)

Plant Protection Research Group