OPTIMATION OF DNA EXTRACTION PROTOCOL OF Elaeidobius kamerunicus

  • Sri Wening Indonesian Oil Palm Research Institute (IOPRI)
  • Agus Eko Prasetyo Indonesian Oil Palm Research Institute (IOPRI)
  • Tjut Ahmad Perdana Rozziansha Indonesian Oil Palm Research Institute (IOPRI)
  • Agus Susanto Indonesian Oil Palm Research Institute (IOPRI)
Keywords: protocol, DNA extraction, Elaeidobius kamerunicus, oil palm

Abstract

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,
material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.

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Author Biographies

Sri Wening, Indonesian Oil Palm Research Institute (IOPRI)
Agus Eko Prasetyo, Indonesian Oil Palm Research Institute (IOPRI)
Tjut Ahmad Perdana Rozziansha, Indonesian Oil Palm Research Institute (IOPRI)
Agus Susanto, Indonesian Oil Palm Research Institute (IOPRI)
Published
2016-08-01